Reduced Senescence and Retained Nuclear DNA Integrity in Human Spermatozoa Prepared by Density Gradient Centrifugation
نویسندگان
چکیده
منابع مشابه
Motility and recovery of alpaca (Vicugna pacos) spermatozoa after centrifugation in a density gradient solution
Background: One of the factors limiting successful processing of alpaca (Vicugna pacos) semen is the viscosity of seminal plasma. The viscous nature of the collected ejaculate has hindered sperm cryopreservation as well as artificial insemination (AI) under field conditions. Aims: The objective of this investigation was to evaluate recovery, motility, ...
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Sperm DNA integrity is essential for accurate transmission of genetic material to offspring. Fragmentation of genomic DNA is an initial hallmark of apoptosis (programmed cell death). The aim of this study was to determine sperm nuclear DNA integrity and mitochondrial function, to quantify possible apoptosis and to investigate any relationship between these parameters. Semen samples (n = 25) wer...
متن کاملcan zeta sperm selection method, recover sperm with higher dna integrity compare to density gradient centrifugation?
background: sperm selection for icsi based on morphology and motility might not be relevant to chromatin integrity. thus sperm selection based on sperm characteristics has been suggested. objective: the aim of this study was to compare the efficiency of zeta method with routine density gradient centrifugation method (dgc) for the selection of sperm with higher dna integrity. materials and metho...
متن کاملvariation of cholesterol, phospholipid and triacylglycerol content in subsets of human spermatozoa isolated by density gradient.
objectives lipid components of spermatozoa have an important role in its functional activity. in this study the concentration of cholesterol, phospholipid, triacylglycerol and total lipid in different subsets of human spermatozoa, isolated as three fractions by a discontinuous puresperm gradient, was determined. methods aliquots of the liquefied semen samples (n=107) were layered on top of the ...
متن کاملeffect of combined density gradient centrifugation on x- and y- sperm separation and chromatin integrity
background: it has been claimed that by using different washing methods, the sperms can be separated according to size, motility, density, chromosomal content and surface markings and charge. these methods also reduce sperm chromatin deficiencies and screen the sperms before applying in assisted reproduction techniques. objective: this study compared simple density gradient methods and a combin...
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ژورنال
عنوان ژورنال: Journal of Assisted Reproduction and Genetics
سال: 2004
ISSN: 1058-0468
DOI: 10.1023/b:jarg.0000040237.47026.0f